· As a powerful tool for fast and precise genome editing, the CRISPR/Cas9 system has been applied in filamentous fungi to improve the efficiency of genome alteration. However, the method of delivering guide RNA (gRNA) remains a bottleneck in performing CRISPR mutagenesis in Aspergillus species. Here we report a gRNA transcription driven by endogenous tRNA promoters which include a …
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Mehr DetailsJody began her entertainment career more than 40 years ago in Michigan. Her ancestors arrived in New York from German and Wales in the1800''s and migrated to Southern Illinois where they became teachers, nurses, lawyers, farmers, pastors, lawmen, with a couple of rogues among them. Jody was born in Ottawa, Illinois just prior to her dad joining ...
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Mehr DetailsShort Interspersed Elements (SINEs) are eukaryotic non-autonomous retrotransposons transcribed by RNA polymerase III (pol III). The 3′-terminus of many mammalian SINEs has a polyadenylation signal (AATAAA), pol III transcription terminator, and A-rich tail. The RNAs of such SINEs can be polyadenylated, which is unique for pol III transcripts. Here, B2 (mice and related rodents), Dip …
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Mehr Details· Tomato is also a model species for basic and applied research in fields, such as genetics, fruit development, and disease resistance, because tomato plants have a short life cycle, are easy to cross and self-pollinate, and are transformed with a high success rate.
Mehr Details· Summary. Eukaryotic cells execute complex transcriptional programs in which specific loci throughout the genome are regulated in distinct ways by targeted regulatory assemblies. We have applied this principle to generate synthetic CRISPR-based transcriptional programs in yeast and human cells. By extending guide RNAs to include effector protein ...
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Mehr DetailsTerminator 3: Rise of the Machines (T3) is een Amerikaanse sciencefictionfilm uit 2003, geregisseerd door Jonathan Mostow. Het is een vervolg op de films The Terminator uit 1984 en Terminator 2: Judgment Day uit 1991.
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Mehr DetailsSandberg BNC Terminator (Network cables). A BNC terminator is used to terminate a bus network. A terminator is required at both ends of a BNC bus network. The terminator
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Mehr DetailsGenetically modified animals are animals that have been genetically modified for a variety of purposes including producing drugs, enhancing yields, increasing resistance to disease, etc. The vast majority of genetically modified animals are at the research stage while the …
Mehr Details· The number of repeats in each TAL effector array ranged from 15 to 20, and the length of the spacers within the target sites were between 15 and 20 bp (). To test activity and specificity, the TALEN pairs targeting the lox3 gene were assessed using a single-strand annealing (SSA)-mediated yeast assay in which green fluorescent protein (GFP) activity served as an indicator of DNA cleavage.
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